Mouse Anti-ERBB2 Recombinant Antibody (MOB-014LC) (CAT#: MOB-014LC)
This product is a recombinant mouse antibody that recognizes human ERBB2. The antibody was purified by affinity chromatography.
Specific Inquiry
WB
Figure 1 Anti-Human ERBB2 Protein Antibody in WB
Western blot analysis of MOB-014LC was performed by loading Recombinant Human ERBB2 Protein (lane 1, Non-Reducing Antigen, 0.2μg; lane 2, Reducing Antigen, 0.2μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MOB-014LC and HRP Goat Anti-Mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human ERBB2 Protein Antibody in ELISA
ELISA analysis of MOB-014LC was performed by coating with Recombinant Human ERBB2 Protein. Then blocked with BSA, and incubated with Anti-Human ERBB2 Antibody (MOB-014LC). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ERBB2
- Species Reactivity
- Human
- Applications
- ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
- Gene ID
- 2064
- UniProt ID
- P04626
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B008(A) | Recombinant Anti-human ERBB2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B008(G) | Recombinant Anti-human ERBB2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B008(T) | Recombinant Anti-human ERBB2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MOB-014LC, RRID: AB_3111465)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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